Ethanolic Extraction, Phytochemical Screening, Antimicrobial and Antioxidant activities of Chamaecyparis obtuse Fruits.

Abstract

This study was carried out in Khartoum State-Sudan, during October (2019). The species Chamaecyparis obtusa (Crippsii) belong to family Cupressaceae   , locally known as (Shagarat Bakhor Alanfar) was chosen because it's using traditionally in treatment of many abdominal disease. Phytochemical activities were investigated to detect the effects of antimicrobial and antioxidant; this plant was collected from Gabal maraha , South Darfur  State, western Sudan. The dried fruits of Chamaecyparis Obtusa was extracted successively with (ethanol), The phytochemical screening carried out on ethanolic  extract of species fruits and showed as table: (1) , that  there were secondary metabolize ( alkaloids ,flavonoids, sterol, tannins  , triturpenes ,cardiac glycoside, phenol and absent of  saponnins). The antimicrobial activity of extract  were evaluated against two standard bacteria (Gram positive; Staphylococcus aureus) and (Gram negative; Escherichia coli,), the results of antimicrobial activities of extract  at different concentrations and inhibition zone were measured in (mm) table (2)  the range of inhibition was found between  15-22mm .Antioxidant was tested and The result of it cited that there is  high  antioxidant activity in the extraction table (3).